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KMID : 0191120100250091305
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Journal of Korean Medical Science
2010 Volume.25 No. 9 p.1305 ~ p.1312
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High Dose Vitamin D3 Attenuates the Hypocalciuric Effect of Thiazide in Hypercalciuric Rats
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Jang Hye-Ryoun
Lee Jay-Wook
Kim Se-Joong
Heo Nam-Ju
Lee Jeong-Hwan
Kim Hyo-Sang
Jung Ji-Yong
Oh Yun-Kyu
Na Ki-Young
Han Jin-Suk
Joo Kwon-Wook
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Abstract
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Thiazide is known to decrease urinary calcium excretion. We hypothesized that thiazide shows different hypocalciuric effects depending on the stimuli causing hypercalciuria. The hypocalciuric effect of hydrochlorothiazide (HCTZ) and the expression of transient receptor potential vanilloid 5 (TRPV5), calbindin-D28K, and several sodium transporters were assessed in hypercalciuric rats induced by high calcium diet and vitamin D3. Urine calcium excretion and the expression of transporters were measured from 4 groups of Sprague-Dawley rats; control, HCTZ, high calcium-vitamin D, and high calcium-vitamin D with HCTZ groups. HCTZ decreased urinary calcium excretion by 51.4% in the HCTZ group and only 15% in the high calcium-vitamin D with HCTZ group. TRPV5 protein abundance was not changed by HCTZ in the high calcium-vitamin D with HCTZ group compared to the high calcium-vitamin D group. Protein abundance of NHE3, SGLT1, and NKCC2 decreased in the hypercalciuric rats, and only SGLT1 protein abundance was increased by HCTZ in the hypercalciuric rats. The hypocalciuric effect of HCTZ is attenuated in high calcium and vitamin D-induced hypercalciuric rats. This attenuation seems to have resulted from the lack of HCTZ’s effect on protein abundance of TRPV5 in severe hypercalciuric condition induced by high calcium and vitamin D.
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KEYWORD
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Hypercalciuria, TRPV5, Sodium Transporters, Cholecalciferol, Thiazides
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